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Once whole-cell configuration was established, cells were held at −80 mV and subjected to various experimental protocols.
After baseline in Ca2+ free solution was established, cells were treated for 30 s with ET-1 (100 nM) or BK (100 nM) and changes in intracellular calcium concentration were analyzed.
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After a whole-cell patch was established, cell capacitance was measured by applying −5 mV voltage steps.
Once the primary cell lines were established, cells from all tissue types (Table 1) grew well and were able to be passaged a number of times.
Once stably transfected Mia.pp32 and Mia.EV cell lines were established, cells were treated with various chemotherapeutic agents from different drug classes (Table 1).
In contrast, once ALI cultures were established, cells looked better defined, smaller in size, and with typical epithelial polygonal shape.
4 days after co-cultures were established, cells were fixed and stained for the indicated proteins (ECAD, SIX2 and SALL1) and for Lotus-tetragonolobus lectin (LTL).
After cell fate is established, cell-cell signaling and intracellular signal transduction pathways instruct cells to undergo cell shape changes.
All the cell lines used in this study are established cell lines.
With regard to PDAC we analyzed a total of 36 samples, 13 of which were established cell lines.
These kinase genes include CCRK which is established cell cycle related kinase [69], MASTL which is a relatively unknown kinase gene associated with mitosis through co-expression analysis and NEK6 which is also an established mitosis related kinase [70].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com