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For example, the GO term for 'GO 0030243; cellulose metabolic process' was enriched in the list of genes with increased transcript levels at 24 h straw but was not enriched in the list at 6 h straw.
Furthermore, with regard to GO terms related to the hydrolysis of lignocellulose, 'GO 0045490; pectin catabolic process' was enriched in the list of genes with increased transcripts at 6 h carbon starvation and 6 h straw.
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For example, retinoic acid targets were enriched in the list of topologically significant proteins.
For the second, we used the DAVID website to identify Gene Ontology terms that were enriched in the list.
In leaf samples, 10 GO terms were enriched in the list of genes up-regulated in response to N limitation for SRG100.
Using IPA, we identified many predicted upstream activators that were enriched in the list of genes with large cross platform correlations (N = 45 genes).
Gene annotation/ontology (GO) analysis of the top upregulated genes revealed an enrichment of genes involved in positive regulation of immune response, while genes involved in lymphocyte maturation and apoptosis were enriched in the list of downregulated genes (Supplementary Fig S6A).
Computer analysis of Medline abstracts (Table 1) showed that immunity and metabolism of reactive oxidative species are prioritized in studies of fish stress and these functional categories were enriched in the list of stress-responsive genes.
To dissect the ontologies that are enriched in the list of DEGs, we employed a top-down screening approach to analyze any disrupted molecular networks on a global level, followed by refined analyses involving specific brain regions or developmental stages.
Q-values were computed using the q-value package in R. Ingenuity Pathway Analysis (IPA; Ingenuity Systems) was used to identify pathways that were enriched in the list of CpG loci with significantly different methylation in YSTs compared with other histologic subtypes of tumors and in immature teratomas compared with mature teratomas.
Inspection of Figure 2 reveals that antigens included in this functional category are enriched in the list of 189 antigens: 12% (23 antigens) as compared to only 2% (~80 antigens out of 3989) in the whole genome (p-value = 1.2 × 10-5).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com