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This GM crop was engineered by introducing into the rice genome a ferritin gene from the common bean, Phaseolus vulgaris, that produces a protein capable of binding iron, as well as a gene from the fungus Aspergillus fumigatus that produces an enzyme capable of digesting compounds that increase iron bioavailability via digestion of phytate (an inhibitor of iron absorption).
A mutation in hpuA (ΔhpuA) was engineered by introducing the chloramphenicol resistance cassette in the gene.
To determine if the levels of expression of M proteins could have an impact on these functions, a recombinant strain that overexpresses emm1 was engineered by introducing a plasmid bearing the emm1 gene (pemm1) into the wild type M1 strain of S. pyogenes.
The truncated chlH gene was engineered by introducing a new NdeI site into the DNA at Met using the following primers: M159TOP, 5′-TGCCGGCTTCCAAGAT CATATGCTCAAACTCC-3′ and M159BOT, 5′-GGAGTTT-GAG CATATGATCTTGGAAGCCGGCA-3′. QuikChange® PCR was carried out according to the manufacturer's protocol (Stratagene), using the T. elongatus cloned pET9aHis6ChlH as the template.
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The third to sixth codons of ndx3, a Nudix hydrolase gene from Thermus thermophilus HB8, were engineered by introducing several silent mutations.
To confirm that these genotypic modifications were consistent with the phenotype expressed by these variants, insertion mutants were engineered by introducing the cat gene, conferring resistance to chloramphenicol, in hpuA and the spectinomycin resistance cassette in hmbR.
That deal was engineered by Mr. Wright.
Desserts are engineered by another local hero.
This is engineered by America".
CL1 deletion constructs were engineered by PCR oligonucleotide amplification.
Recently, carbon-based materials such as reduced graphene oxide (rGO), graphene oxide (GO), and graphene are being more frequently used for various applications such as redox catalysts or fuel cells because they can be easily engineered by introducing functional groups [1 4].
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