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The reaction was ended by adding 2 M sulfuric acid (100 µl/well).
The reaction was ended by adding 600 μL of 200 mM Na2CO3.
The reaction was ended by adding 35 µL of stop solution (0.5 M EDTA pH 8.0) and incubating at 95° for 10 minutes in order to inactivate the enzyme.
The response was ended by adding of 50 mmol/L HEPES buffer containing 5 mmol/L EDTA.
Incubation was ended by adding 20 μl of 2× electrophoresis sample buffer and boiling for 5 min.
The plate was developed with TMB detection kit (Amersham Biosciences) for 8 10 min at 23°C and the reaction was ended by adding 1 M H3PO4.
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Incubations were ended by adding 57 μl of 100% trichloroacetic acid (5% final concentration; TCA, Fisher Scientific) and frozen for storage prior to processing by the method of Smith and Azam (1992).
The experiments were ended by adding ice-cold 5% trichloracetic acid (TCA) and the wells were washed twice with TCA, then precipitated DNA was dissolved in NaOH 10% solution and counted on a liquid scintillation counter (Wallac, 1409 DSA).
According to Craig, my friendly bait-slinging salesman, minnows' lives are best ended by adding salt to their water.
Double-stranded cDNAs were then blunt-ended by adding 10 units of T4 DNA polymerase (New England Biolabs) and continuing the incubation for 10 minutes.
Sheared DNA was end-repaired by adding End Repair Mix (Illumina) and incubated at 30°C for 30 min.
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CEO of Professional Science Editing for Scientists @ prosciediting.com