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Furthermore, due to its metabolic activation, 35b was efficiently processed in liver cells compared to other cell types, including human cardiomyocytes.
However, when expressed as a fusion with the leader sequence from the ompA gene, hexahistidine-tagged PNGase F was efficiently processed and exported to the E. coli periplasm.
As expected, the endogenous procaspase-3 in the control lysates was efficiently processed by exogenous caspase-8.
Therefore, it is reasonable to propose that the N-terminal methionine of the rArtinM was efficiently processed in E. coli BL21-CodonPlus DE3 -RP BL21-CodonPlus DE3 -RP
By contrast, the benzoylated arginine series was efficiently processed, indicating that an N-terminal amide is critical and sufficient for substrate recognition.
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Analysis of the primary miRNA transcript level using RT-qPCR showed an enrichment of these transcripts for the chimeric constructs, suggesting that the murine flanking regions used in this study result in structural changes that cannot be efficiently processed by Drosha/DGCR8 in the nuclear processing step.
Indeed, in a strain carrying a temperature-sensitive rsp5 allele, Def1 failed to be efficiently processed in response to 4-NQO at the restrictive temperature, whereas Def1 processing and accompanying Rpb1 degradation was observed in the WT control strain.
As the removal of terminal introns in spliced genes generally results in a significant inhibition of 3′end processing, it is unclear how naturally intronless pre-mRNAs can be efficiently processed at their 3′end.
User revocation can be efficiently processed and is suitable for e-health cloud application.
The choice of patterns and symbols is important so that visual queries can be efficiently processed by the intended viewer.
Consequently, SPARQL queries can be efficiently processed regardless of where the variables occur (subject, property, object) since one of the indexes will be applicable.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com