I'm still attracted by the notion, though, that his outsized supply of energy and drive and competitiveness was drained off at table, in the savoring of a decent Burgundy or the perfect crab cake.
The biodiesel separation was carried out by decantation as the glycerin was drained off while the biodiesel remained.
After overnight soaking, excess water was drained off, left in full sunlight and occasionally weighed until the moisture content was brought to 65 70 % (Iqbal et al. 2005).
The supernatant was then transferred into a separating flask, and the lower phase (chloroform phase) was drained off into a 250-ml Erlenmeyer flask containing 4 g anhydrous sodium sulfate and shaken vigorously.
Excess was drained off and coverslips fixed in 0.5% (v/v) gluteraldehyde for 15 min. Coverslips were washed 3 times in PBS, incubated in ice-cold NaBH4 (3 min) with agitation, washed with PBS, and stored in 70% ethanol.
After overnight, medium was drained off with the addition of viral inoculums to the seeded-cells.
