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Glycidyl azide polymer was dissociated with infrared radiation at 1.064 μm from a Nd YAG laser; gaseous products were detected in situ with a Fourier-transform infrared spectrometer.
The tissue was dissociated with 1.0 mm glass beads using a mini-beadbeater (Biospec) in Trizol (Invitrogen) and RNA extraction was performed according to the manufacturer's instructions.
Calcium was dissociated with trichloroacetic acid and reacted with o-cresolphthalein complexone, forming a purple color which was measured spectrophotometrically at 575 nm using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA).
Tissue was dissociated with silicone-coated Pasteur pipettes and centrifuged at 1000 rpm at 4°C for 10 min. Dissociated neurons were plated on glass coverslips pre-coated with Matrigel (BD Biosciences) at a rough density of approximately two hippocampi per 24 coverslips.
Tissue was dissociated with 0.1% Trypsin (Worthington) and 0.1% DNAse1 (Roche).
The epithelial layer was dissociated with PBS EDTA (5 mℳ) and cells were detached by scraping (Rocks et al, 2008a).
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Samples from human tumors were dissociated with the Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ, USA).
Therefore atrial tissue is dissociated with collagenase and the dissociated cells of interest (cardiac fibroblasts) adhere rapidly to cell culture plates, for example, within 3 h of plating.
Confluent tumor cells (80 90 %) were dissociated with 0.05 % trypsin-EDTA and neutralized with culture medium.
Like me, they were conditioned to despise a part of who we are, and to be proud to be dissociated with aspects of identity.
After 6 days of culture the cells are dissociated with TrypLe and analyzed by flow cytometry.
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