Brains were dissected between 11 and 14 months of age, fixed in 10% formalin and paraffin embedded.
Embryos were dissected between E9.5 and E11.5, and immediately fixed in 4% paraformaldehyde and 5% glutaraldehyde as described previously (Schmidt et al., 2010).
Another approach to test the validity of a model of speciation is to make predictions regarding the type of results that could be expected from the model proposed, and then to check whether those predictions hold up when the genetic source of reproductive isolation is dissected between closely related species.
LV anterior or posterior papillary muscle was dissected free, mounted between two spring clips, and placed vertically in a chamber containing Krebs–Henseleit solution at 28°C and oxygenated with a mixture of 95% O2 and 5% CO2 (pH 7.38).
Left ventricular anterior or posterior papillary muscle was dissected free, mounted between two spring clips, and placed vertically in a chamber containing Krebs-Henseleit solution at 28°C and oxygenated with a mixture of 95% O2 and 5% CO2 (pH 7.38).
Every nuance of the company's accounting techniques was dissected, from transfer pricing between subsidiaries to the apportionment of overheads and price setting.
A section of conduit artery (~500 μm diameter), from half way down the main artery running the length of the left lung lobe, was dissected out and mounted between two pins in a small vessel myograph chamber (Danish Myotechnology).
The muscle of interest (m. rectus femoris, m. gastrocnemius or m. gluteus maximus) was dissected out and suspended between a fixed clamp at the base of an organ bath and a Grass FTO3C (USA) force-displacement transducer.
Afterwards, the shoulder was dissected to study the relationship between all portals and nerves.
To illustrate this fact, and to confirm the developmental timing of our genotype under study (w 1118 ), wing tissue was dissected at several developmental time points between the late third larval instar (L3) and 36 h after puparium formation (APF).
