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Liver was dissected as rapidly as possible, immediately frozen in liquid nitrogen and stored at −80°C.
After completion of the protocol, the proximal (i.e., oxidative) and distal (i.e., glycolytic) portion of gastrocnemius medialis muscle [ 28] and soleus muscle (in Protocols 1 and 2 only) was dissected as rapidly as possible and snap-frozen in liquid nitrogen.
The striatum was dissected as follows.
The cerebellum was dissected as a whole.
The olfactory epithelium of OMP-GFP mice was dissected as described previously.
Renal artery was dissected as in TX-groups and ligated using a microvascular clamp.
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Primary care physicians need to be dissected as well, handing off routine care such as treating earaches and performing camp physicals to retail clinics and assigning the care of chronically ill patients to disease management networks.
The abdominal/distal oesophagus is dissected as far as possible toward the distal end.
Subsequently, the abdominal oesophagus is dissected as far as possible toward the distal end.
The infarct core and remaining ipsilateral tissue (designated as penumbra for simplification) were dissected, as well as the contralateral cortex, from coronal brain sections covering the majority of damage.
For endoderm dissection, E7.5 embryos were dissected as stated before and the embryonic fragments were incubated for 10 15 min at 4°C in a solution of 0.5% trypsin and 2.5% pancreatin in PBS.
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