Your English writing platform
Discover LudwigSuggestions(2)
Exact(35)
The cell suspension was disrupted using Constant Cell Disruption Systems Constant Systems Ltd , Warwick, UK).
To prevent the degradation of l-tryptophan and 5-HTP, the tnaA gene of E. coli BL21 (DE3) was disrupted using the Red/ET recombination system.
Fresh mycelium was disrupted using Fastprep with glass beads and 400 μL DNA extraction buffer (0.1 M Tris HCl pH 8.0, 1.2 M NaCl, 5 mM EDTA).
Transformants (His+) were further subjected to colony direct PCR to check whether the FOB1 gene was disrupted using an oligonucleotide primer pair FOB1_deletion check-F and FOB1_deletion check-R.
The tryptophanase gene was disrupted using the λ red recombination system, since tryptophanase is postulated as an initial enzyme for the degradation of l-tryptophan and 5-HTP in E. coli.
For that, fresh mycelium was disrupted using MP lysing matrix C tubes and 400 μl DNA extraction buffer (0.1 M Tris HCl pH 8.0, 1.2 M NaCl, 5 mM EDTA).
Similar(25)
H3mm7 genomic loci were disrupted using the CRISPR/Cas9 system.
Bacteria were disrupted using a French press before centrifugation at 12,000 g for 90 min at 4 °C.
Bacteria were disrupted using a French press before centrifugation at 12000 g for 90 min at 4 °C.
Cells were disrupted using a constant flow cell disruptor, and cell debris and inclusion bodies were removed by centrifugation at 10,000 g for 30 min.
The pellet was resuspended in buffer A (to which 1 mM EDTA was added) and cells were disrupted using a cell disruptor (Constant Systems).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com