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In the 31d group, a large number of leukocytes were infiltrated, and the endometrial tissue structure was disintegrated with erythrocyte leakage (Fig. 2F).
For western blotting, frozen tumor tissue (about 100 mg) was disintegrated with a Sonifer Cell Disruptor in lysis buffer containing a cocktail of protease inhibitors (Roche Biochemicals); 50 to 100 μg of the isolated protein was separated by electrophoresis in a 10 to 20% Tris-glycine gel and transblotted to Immobilon-P membranes (Millipore, Bedford, MA, USA).
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At a temperature of about 50°C, only a broad relaxation mode was observed indicating that intermolecular aggregation is disintegrated with increasing temperature, but the intramolecular aggregation of an isolated chain still exists due to the poor affinity of DOA to PVC chains in this temperature region.
The pellet was resuspended in distilled water and approximately 0.2 × 10 cells were disintegrated with a FastPrep FP120 (BIO101) (6x20 s, speed 6.0) using glass beads.
The cells were disintegrated with the ball mill Mikro-Dismembrator U (B. Braun Biotech) in 400 μL Buffer RLT and afterwards resuspended in 1.4 mL Buffer RLT and 2.7 mL pure ethanol.
The NgSdhB-GFP signal was not affected when the mitochondria were treated with trypsin; however, the signal disappeared after trypsin treatment when the organellar membranes were disintegrated with a detergent, which is consistent with the presence of NgSdhB-GFP in the mitochondrial matrix (fig. 2 B).
Wood cellulose was disintegrated and washed with distilled water before use.
The dried product was disintegrated in a mortar and fractioned with the use of mesh sieves.
It was clear that the system was disintegrating and – with hindsight– that meant the Berlin Wall had to fall.
However, while his career flourished, his marriage was disintegrating, and Sara returned to Britain with their three children.
"Our relationship was disintegrating.
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