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A Filtrate of 100 μL was diluted with 600 μL of the sample dilution buffer.
The reaction mixture was diluted with THF and precipitated in methanol for several times.
This solution was diluted with acetonitrile to 50 mL total volume.
The digested solution was diluted with deionized water to a final volume of 10 mL.
After completion, the reaction mixture was diluted with water and extracted with EtOAc (x3).
Clarified cell culture medium was diluted with 1/10 volume 10× PBS pH 7.4.
After completion, the reaction was diluted with water and extracted with EtOAc (x3).
The extracted complex was diluted with ethanol and injected into a graphite furnace.
After centrifugation, the surfactant-rich phase was diluted with 0.25 mL acidic ethyl alcohol.
The extract was diluted with methanol.
The concentration of MNFs was diluted with deionized (DI) water.
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