Two days after plating, cells were differentiated using "Differentiation Medium" (catalog # DM-2, ZenBio) and considered as Day '0'.
We first examined expression of total tau and pS199/202 tau in RGs that were differentiated using the cortical neuron differentiation protocol.
Then, the filtered signal, f[n] (n denotes sampling points), is differentiated using five-point first-order differentiation to provide information about the slope of the QRS complexes and reduce the influence of the P and T waves [43].
The transit and eclipse spectroscopy method, whereby the signal from the star and planet are differentiated using knowledge of the planetary ephemerides, allows us to measure atmospheric signals from the planet at levels of at least 10−4 relative to the star.
rMAPC were differentiated using the same protocol, except that the differentiation was done without FCS and the starting cell density was 50,000 cells/cm2 (as described in [38]).
Two days after reaching confluence, cells were differentiated using adipocyte basal medium (ZenBio) and a standard differentiation protocol.
For in vitro differentiation of Myf5-LacZ-NERT ESC and Runx1-Venus-NERT ESC, ESC were differentiated using the hanging drop method as described [59].
From there, melanin subtypes can be differentiated using post-processing techniques such as principal component12 or phasor analyses17.
Two populations of P. japonicus could be differentiated using one of the ESTs.
The iNGN cells were differentiated using 0.5 µg/ml doxycycline (Sigma, D9891) in mTeSR1 medium (Stem Cell Technologies, 05850), supplemented with penicillin/streptomycin (Thermo Fisher Scientific, 15140122) as previously described5.
