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After consistency between the two methods was determined, subsequent samples were read by the ARIS exclusively.
Nuclear (Fn) and cytoplasmic (Fc) fluorescence was determined subsequent to the subtraction of autofluorescence (also quantified), and used to derive the nuclear to cytoplasmic ratio (Fn/c), a parameter that enables differences in expression levels between individual transfected cells to be standardized.
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Once optimal specimen collection type and analysis methods have been determined, subsequent testing will be streamlined.
The acetylcholinesterase (AChE electrical eel, Type VI-S, EC 3.1.1.7; Sigma) and butyrylcholinesterase (BuChE equine serum EC 3.1.1.8; Sigma) inhibitory activities of Ir.Cr were determined subsequent to minor modification of previously reported spectrophotometric method [ 42] by using 96-well plate reader Synergy HT, Biotek, USA, taking eserine (0.25 mM per well) as standard inhibitor.
Hence, an optimal step size μ for the variants of the GFDAF algorithm was determined by subsequent increasing μ until no further improvement in ERLE was noticeable.
In this study, the ability for CO2 to absorb in AN copolymers containing 85 98 mol% acrylonitrile was determined, and subsequent viscosity and equivalent processing temperature reductions were evaluated.
It was determined in subsequent research that these strobing light effects cause some individuals to have epileptic seizures, even if the person had no previous history of epilepsy.
Specificity of the reactions was determined by subsequent melting curve analysis.
quinquefasciatus EDS39158 sequences was determined by subsequent expression (Table S1) and biochemical analysis of their recombinant proteins (not shown).
The species identity was determined by subsequent amplicon sequencing.
The RT-PCR was run for 40 cycles and specificity of the reactions was determined by subsequent melting curve analysis.
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