For each isolate, the MICCIP was determined in triplicate and the median MICCIP was recorded.
The moisture was determined in triplicate using 5 g of the oil.
The percentage extraction of CdCl2.H2O and ZnCl2 using SF was determined in triplicate under static conditions.
Blood glucose was determined in triplicate by the glucose oxidase method using Glucose GOD-PAD kit (Rongsheng Biotech, Shanghai, China).
The gloss was determined in triplicate per time point, and the apparatus was repositioned in between each analysis.
In each case the CFU/ml was determined in triplicate by plating 100 μl aliquot from serial dilutions on non-selective blood agar plates.
Cell dry weight was determined in triplicate by filtering 5 mL of the culture on a pre-weighed 0.45 μm pore size Supor® membrane disc filter (Pall Corporation, Port Washington, NY, USA).
Disrupted cells were centrifuged (4,816 × g, 30 min, 4°C) and the protein concentration of the crude extract was determined in triplicate with the Bio-Rad Protein Assay (Bio-Rad Laboratories, HercUSAs, USaccordinging to the manufacturer's instructions.
The bacterial cell dry mass was determined in triplicate by pipetting 1 ml washed cells in predried reaction tubes (60°C, 24 h), centrifugation (24,725 × g, 20 min, 4°C), drying of the cell pellets (60°C, 24 h) and gravimetrical quantification.
Each sample was determined in triplicate.
Cell proliferation was determined in triplicate.
