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Oxidative damage was determined by quantifying nitrotyrosine content.
The oxidative stress induced by H2O2 was determined by quantifying the intracellular reactive oxygen species production using DCF-DA staining.
The extent of mineralization of lindane was determined by quantifying the release of inorganic chloride ions and analyzing the percent residual lindane in the medium.
Refolding efficiency was determined by quantifying precipitation (O.D. 405 nM) and ATPase activity.
Differentiation was determined by quantifying glycerol-3-phosphate dehydrogenase activity as described [25].
Synapse formation was determined by quantifying co-immunostaining between the presynaptic marker synaptophysin and contacting axons of transfected COS7 cells.
Total spot area or total spot intensity was determined by quantifying the GFP-positive spots in the GFP channel.
The cell cycle distribution of the cell lines was determined by quantifying DNA content, after PI staining (Figure 5).
The viability of mesencephalic cell cultures, known to be rich in dopaminergic neurons, was determined by quantifying tyrosine hydroxylase (TH) immunoreactivity after exposure to LPS.
AR expression was determined by quantifying the sum of pixel intensities in the AR channel within the defined cell region for each cell.
Q344ter transgene expression level was determined by quantifying the mutant-to-total transcript ratio in transgenic Q344terrho+/− mice at postnatal days 30 (p30).
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