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The samples were then boiled for 20 min, and the total conductivity was determined again (C2) after cooling to room temperature.
This study, however, was performed in a xenograft model in SCID mice, and therefore, the metabolic stability of both radiotracers was determined again in SCID mice and results were compared with previously obtained rat data [19, 20].
The protein concentration was determined again by measuring the absorbance at 280 nm and using the molar extinction coefficient of the S. cyaneus laccase coding sequence including the C-terminal His6 tag.
After the first hemodilution step, Hct was determined again, and ABG was analyzed.
These experiments were then repeated in intrathecal RTX-injected animals and the PWL was determined again.
A high-resolution crystal structure of the complex was determined, again showing the 2∶2 complex seen before, and extensive disorder is seen in the hydrophobic pockets of CaM that interact with the peptide.
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The changes in morphology, crystal structure and optical properties were determined again using the methods outlined above.
In this way, the number of clusters is reduced, the new clusters are resolvable and meaningful CRLBs can be determined again.
The diffusion coefficient D n of clusters of size n in solution will be determined again using Stokes Eq. 12, which describes experimental data.
Since this probability depends on the assumed number of latent groups and therefore has to be determined again and again in the course of the estimation, a large number of observations are necessary to obtain statistically significant results.
Secondly, if ( P_{TUi}^ > 0 ) and ( P_{TUi}^ > hat{P}_{TUi}^{disch} ), ( P_{TUi} ) calculated by (1) is determined again by using (2) and (3) based on the current allowable discharge power constraint for each transformer unit.
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