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The amount of rhynchophorol recovered was determine using a gas chromatograph (Clarus 680), coupled to a mass spectrometer detector (Clarus 600C), with an ELITE-5MS capillary column (Perkin Elmer/USA).
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Creatinine concentration was determined using a creatinine standard curve.
Statistical significance was determined using a Student's t test.
Protein concentration was determined using a Pierce BSA assay kit (Thermo Scientific).
LDH activity was determined using a LDH Cytotoxicity Detection Kit (Takara, Ohtsu, Japan).
Cell number was determined using a Scepter Cell Counter (Merck Millipore).
Absorption was determined using a microplate reader at 450 nm.
The fluorescence quantum yield of miRFP670nano was determined using a Nile blue dye as a standard.
Light dose was determined using a SE-9087 DigitaLightht Meter (Extech Instruments).
pH of the solution was determined using a pH meter pp-15 (Sartorius, Germany).
The protein concentration was determined using a bicinchoninic acid assay kit (Thermo Fisher Scientific Inc.).
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