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No significant difference in Cq values was detected when using one-step and two-step multiplex RT-qPCR detection formats.
In the hybrid ears, nearly 10% kernel abortion was detected when using CAU5 as the male, while almost no aborted kernels were detected when using CAU5 as the female (Supplementary Fig. 1).
A strong negative buffer effect was detected when using 50 mM Na2HPO4/KH2PO4 (pH 5.0 to 7.0).
Furthermore, and as expected, no inhibition was detected when using the negative control water (data not shown).
In this work, a new structure of molecular cluster composed of melamine and cytosine was detected when using cytosine-melamine binary system at 1-octanole/graphite interface.
Since no carryover or contamination between wells was detected when using the automated liquid handler, individual wells of a single 96-well plate were subsequently used to replicate experimental conditions and provide positive and negative experimental controls.
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Achievable serum concentrations of succinate were about 1 mM and no antitumour activity of succinate was detected when used in this way.
This system allowed us to demonstrate that recombination activity could be detected when using a dsattC, even if this process was strongly increased when the bsattC was provided.
No significant differences were detected when using the criteria mentioned above for the time-step parameter η and relative energy conservation.
The β-haemolysin gene hlb can only be detected when using random primer directed amplification, suggesting the presence of an allelic variant.
However, because there were only 6.6% of persons with severe obesity in the sample, it is possible that the relationship could be detected when using a larger sample size.
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