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The image was detached into non-overlapping 8×8 patches.
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Then, each low-resolution frame I ′ is detached into N non-overlapping patches {P i |i=1,2,⋯,N} (see Fig. 2).
Adherent cells have to be detached into a single cell suspension prior to fixation.
However, since flow cytometry is applicable only to suspended cells, adherent cells have to be detached into a single cell suspension prior to the analysis, which possibly perturbs the signaling activity of interest.
Briefly, mitotic cells were detached into the media by gentle agitation of the plate and the remaining adherent cells were collected separately after trypsinization.
Cells were treated with 20 ng ml−1 colcemid for 4 h and mitotic cells were detached into the medium by tapping the flask.
However, these methods require cells to be detached into suspension prior to analysis, an event which could activate many signaling pathways and perturb the biochemical process to be studied.
The regiment remained fragmented into May, for once training was completed, another squadron was detached and sent into Cassino itself to man tanks which had been effectively setup as pillboxes.
In 1802 Austrian Frickthal was added to the republic, and Valais was detached and made into an independent republic (later, in 1810, annexed to France).
A 75% confluent monolayer was detached with trypsin and dissociated into a single cell suspension for further cell culture.
Neuroepithelium was detached from the underlying tissue and mechanically dissected into single cells.
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