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sipD gene was deleted using one-step deletion strategy [59].
Measurements in our laboratory found no BrdU-positive fiber cells at P3 when the growth factor receptor gene Tgfbr2 was deleted using the LeCre transgene (see Methods), suggesting that deletion of Trp53, not simply LeCre expression, was the cause of the excess proliferation (Claudia M. Garcia, unpublished results).
To test a putative role of Eng2 for morphogenesis, ENG2 was deleted using the strategy outlined in Fig. 5. Growing the deletion strain in various media (1% yeast extract with 3% glucose, Sabouraud's medium, RPMI, and minimal medium) in solid and liquid cultures at different temperatures did not show a growth phenotype.
d, The ATAC day 7 peak was deleted using CRISPR Cas9 to generate a heterozygous knockout (ATAC-KO) for the region downstream of TFAP2C and to determine the effects of its loss on TFAP2C expression.
For ExonA-Rev (without CMV or SV40 promoters), the CMV promoter was removed from ExonA-Rev using BglII and BamHI digestion followed by blunt-end ligation, and the SV40 promoter was deleted using DraIII and BstZ17I digestion and ligation.
The kanamycin-resistance gene was deleted using FLP recombinase by electroporating pCP20 (CmR) [70], [71].
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Observations with missing data will be deleted using listwise deletion at either the MDM creation stage or when the analysis is run.
Genes were deleted using a markerless gene-deletion system as described previously (Sun et al. 2013).
In addition, items that still misfitted after the collapsing, were deleted using a step-wise top-down deletion strategy until the remaining items fit the model [ 83].
To construct a single-deletion "starter" strain library, the TF-encoding open reading frames were deleted using a PCR-based gene replacement strategy conferring uracil prototrophy or kanamycin resistance.
Single FoVs can only be deleted using the ObsVis window, not in ds9.
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