Sentence examples for was deleted by using from inspiring English sources
Afterwards argG was deleted by using pK19mobsacB∆argG to obtain CIT0.
The asdA gene was deleted by using the described suicide vector technology.
In C. acetobutylicum, spo0A was deleted by using a replicative plasmid pETSPO capable of integrating into the chromosome through two rounds of crossover selection [36].
The gfa1 gene of the fission yeast Schizosaccharomyces pombe was deleted by KanMX6-mediated gene disruption and the Cre-loxP marker removal system, and the glmS gene of Escherichia coli was deleted by using λ-Red mediated recombinase system.
Some form features were deleted by using an attribute reduction algorithm based on Rough Sets Theory (RS T.
To test whether the suppression of the tpp1− phenotype is due specifically to the loss of stpA function, we constructed a double mutant strain, tpp1flox− /stpA−, in which the entire stpA open reading frame had been deleted by using homologous recombination.
For the evaluation of its industrial applicability we tested this system in heterologous and homogenous SED1-disruptants of sake yeast, a diploid S. cerevisiae strain, in which either the SED1 ORF or the complete gene including the promoter was deleted by use of the high-efficiency loss of heterozygosity method.
To generate the cytosol-targeting ELuc expression vector pCMV-Flag::ELuc (cyto), the ELuc cDNA in which the peroxisome-targeting signal (PTS; Ser-Lys-Lue) at the extreme C-terminus was deleted by PCR using pBlue-ELuc as the template, and the product was ligated to the EcoRV/XhoI site of pCMV-Tag2B.
The miR-205-5p miR-205-5p miR-205-5p (5 bpredicted was deletarget PCR usite the following primers: Her2MutFw 5′- gccctgatgtgtcctcagggagcaggcc-3′ and Her2MutRev 5′-tgatgccagcagaagtcaggcctgctcc-3′ with QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA).
saeRS was deleted by allelic exchange using the pMAD vector, as described by Arnaud et al [36].
On this strain, SWF1 ORF was deleted by gene replacement using S. pombe HIS5 as a selection marker (oligos swf1 KO 5' and swf1 KO 3') to generate a bsd2Δ tul1Δ swf1Δ strain.
