The mobile phase was degassed using a model 1100 micro vacuum degasser (Agilent Technologies, Santa Clara, CA, USA), and the analyses were controlled by the MassHunter Workstation software (Agilent Technologies) or LCMS solutions software (Schimadzu Corporation, Kyoto, Japan).
The ampoule was degassed using nitrogen gas continuously about 15 min after which it was sealed.
The water for the preparation of standards was degassed using ultrasonication (10 min) followed by purging with N2 (10 min at 200 mL/min).
The resulting mixture was degassed using an ultrasound bath (Elma S 30 H, Elmasonic) for 15 minutes at 40°C and finally TEA was added under vigorous stirring.
It was degassed using vacuum line.
Briefly, 10 mL of PBS (pH 7.4) was degassed using an Ar purge for 60 min.
For the growth of a 20 nm-thick POEGMA brush: a solution of CuBr2 (9 mg, 40 μmol), bpy (160 mg, 1.0 mmol) and OEGMA (6.3 g, 17.5 mmol) in water/ethanol 4/1 (15 mL) was degassed using nitrogen bubbling for 30 min.
The samples were degassed using vacuum under the temperature of 90°C for 3 h.
Mobile phases were degassed using Merck L 7612 solvent degasser.
Samples were degassed using a thermovac (MicroCal Inc).
