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EV71 virus was cultivated using serum-free VP-SFM medium in a BIOFLO 310 bioreactor (NBS, US) based on the microcarrier cell culture bioprocess previously reported [15], [23].
Three quarters of an acre of impacted earth destined for the potatoes was cultivated using a mattock and a crow bar; he cuts the grass with a scythe; he'd prefer to use a hoe over a tractor.
Hydrocarbon-degrading bacteria Bacillus subtilis N3-1P was cultivated using different DHs of hydrolysate.
As controls, a ΔfrmA strain containing the empty pTrc99A vector (strain Ec-ΔfrmA-pTrc99A) was cultivated using the aforementioned media.
E. aerogenes ATCC 13048 was cultivated using sterilized regular tryptic soy broth (n-TSB) and agar (n-TSA).
A few decades ago, the crop was cultivated using rudimentary practices, but today farmers use modern farming technologies including herbicides due to several factors.
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Usually, microalgae can be cultivated using four types of cultivation methods such as phototrophic, heterotrophic, mixotrophic, and photoheterotrophic [ 37] cultivation methods (Table 2).
In the kitchen garden on Bakers Hill, fruit and vegetable varieties recorded by White are cultivated using period tools and methods.
The best are cultivated using organic methods, with crab shells as fertiliser to nurture the climbing stems, and fallen branches as supports.
On another floor, they are experimenting with new forms of aquaponic technology in which fish, vegetables and herbs are cultivated using the same water.
In the 17th and early 18th centuries, tobacco and, later, cacao were cultivated, using Trinidadian Indian labour, but after a disastrous failure of the cacao crop in the 1720s, the industry declined.
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