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In order to create the single direction F1 cross (K × LJ), milt was cryopreserved from the earlier spawning desert strain (LJ) and used to fertilize eggs from montane fish (K) about 4 5 weeks later.
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Thus, the cells were cryopreserved from 4th to 6th passages.
Human islets were cryopreserved from 1991 to 2001 and thawed between 2012 and 2014.
Cells were isolated from human lipoaspirate tissue, then they were expanded for one passage in MesenPRO RS Medium (Invitrogen, Life Technologies, Foster City, CA, USA), a low (2%) serum concentration medium that reduces ASCs doubling times and finally they were cryopreserved from primary cultures.
Children have been born from embryos that were cryopreserved for more than 8 and 12 years [ 33].
Sperm from these males was cryopreserved and their carcasses were used for the preparation of genomic DNA as described [ 16].
Semen from 115 men was cryopreserved and after a median follow-up time of 10.1 years, 33 men have utilised stored semen (actuarial rate 27%) and nine partners have become pregnant resulting in 11 live births and one termination for foetal malformation.
Objective: To report successful pregnancy from embryos that had been cryopreserved for 8.9 years.
One blastocyst was cryopreserved.
One non-transferred blastocyst was cryopreserved.
This product was cryopreserved until its use.
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