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Keratin within PCL was crosslinked to decrease its solubility, before coating of CaP.
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Then the composite SPPO membranes were crosslinked to decrease the methanol permeability and increase the mechanical properties, and the crosslinking time was controlled to determine the optimal crosslinking time.
Briefly, DNA was crosslinked to protein with formaldehyde.
To clarify this question we compared DNA that was crosslinked and reversed with DNA that was not crosslinked.
Solomon and Varshavsky [2] found that purified DNA binding proteins were indetectably crosslinked to DNA in vitro, suggesting that protein-DNA crosslinks are far less frequent than inter- and intra-molecular protein crosslinks.
For example, if a protein is crosslinked and contains only two tryptic peptides, one will be missed due to the crosslink modification.
As the nascent chain was extended beyond 96 residues, crosslinking to Sec61α decreased abruptly and was undetectable by 126 amino acids.
The apparent diffusion coefficients were found to decrease with increasing crosslink density, while they increased with increasing film thickness.
Crystallinity and swelling were shown to decrease with the increasing content of the crosslinking agent.
Only recently has living/controlled polymerization been exploited to decrease heterogeneity and substantially improve the efficiency of the imprinting process for both highly and weakly crosslinked imprinted polymers.
The probes were then UV crosslinked to the membrane.
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