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Bacterial colony was counted by using colony counter under optical microscope (×40).
Hoechst-stained nucleus was counted by using the "Analyze particles" tool in ImageJ software.
The number of migrated cells was counted by using light microscope at high magnification field (20×).
Radioactivity was counted by using liquid scintillation counter (Perkin Elmer).
The number of circular tubules was counted by using Image-Pro® Image-Pro®Media CyberMetics Inc., Bethesda, MD) software.
Five random fields of view were captured per transwell insert, and the number of cells that had migrated to the bottom side of the membrane was counted by using the particle counting module of ImageJ after size and pixel intensity thresholding.
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Cell numbers were counted by using an automated cell counter (Bio-Rad, USA).
And the NCBC-parameters in a time-period are counted by using all of the PEVs that are plugged into the grid during that time-period.
The colonial and filamentous microalgae such as Microcystis sp., Merismopedia sp. and Oscillatoria sp. have been counted by using plating method (Stanier et al. 1971).
Numbers of cells were counted by using particle count filter set with size limitation.
Cell viability and dead cells were counted by using Guava ViaCount reagent (Gevara Technologies) cell counter (ViaCount).
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