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For each replicate, the radioactivity of the samples was corrected against a blank which corresponded to the pre-fixed sediment cores submitted to the protocol described above.
Gene expression was corrected against GAPDH and GUS mRNA level in each sample.
The EGFR mRNA expression level was corrected against GAPDH mRNA in both cell lines.
The selected genes relative expression was corrected against GAPDH and GUSB genes as endogenous controls.
Normalization for candidate gene expression was corrected against housekeeping gene expression; each candidate gene was divided by its normalization factor for each mouse muscle sample.
In analysis, conductance derived CO was corrected against CO measured by thermodilution measurements of cardiac output by a thermodilution computer (Vigilance, Edwards Lifesciences, Irvine, CA, USA) at each temperature step.
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Peak areas were calculated in El Maven (https://elucidatainc.github.io/ElMaven/) and stable isotopic measurements were corrected against the naturally occurring isotopes for each metabolite measured.
The hypocretin contents were corrected against protein concentrations.
Urinary amino acid concentrations were corrected against creatinine values.
The assay results were corrected against β-gal activity assayed using Promega β-gal reporter kit.
Signal intensities were corrected against the membrane background.
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