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The recording solution was continuously bubbled with 95%O2/5%% CO2 at source.
During the electrochemical measurements, the electrolyte in electrochemical cell was continuously bubbled with N2, and current densities were normalized with the geometric surface areas of the electrodes.
Experiments were carried out in an especially designed glass vessel in which helium gas was continuously bubbled through the solution to create uniform concentration and to remove hydrogen gas from the surface for the case of hypophosphite-based procedure.
The ACSF was continuously bubbled with 95% O2/5% CO2.
The ventricle was incubated in buffer containing Lucifer yellow (2.5 mg/ml) and rhodamine-conjugated dextran (2.5 mg/ml), which was continuously bubbled with O2, for 20' at 37°C.
Isoosmotic oxygen-and-glucose deprivation was achieved by substituting sucrose for D-glucose and bubbling solution with 95% N2/5% CO2 for at least 30 min. The same OGD solution was continuously bubbled with the same gases during slice recording in the chamber.
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Horizontal slices (222 µm) were taken using a Leica vibratome and allowed to recover for at least 30 minutes prior to use in Krebs with MK-801 being continuously bubbled with 95/5% O2/CO2.
The foamability of aqueous suspensions of proteins and fat particles containing different nonionic surfactants relevant to ice cream mix, through which air is continuously bubbled in a foam column, is investigated in terms of the growth of the foam until steady state is reached.
As water steam is continuously bubbled into a jujube extract solution in the three-necked, round-bottom flask, the vapor captures the volatile components of the jujube extract.
HEPES-buffered solutions were continuously bubbled with O2.
The header tanks were continuously bubbled with air to aid mixing and to maintain dissolved oxygen (DO) >90%.
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