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We are, however, unable to account for over-the-counter treatments and short courses of treatment in hospital which have not been continued subsequently in the community.
Prior to addition of a stock solution, the matrix was kept at a stable temperature for 15 min in a homemade thermostat with a precision of ±0.2 °C and after addition, thermostating was continued for another 15 min and, subsequently, the fiber was immersed in so prepared standard solution.
Subsequently, anesthesia was continued with 24 mg/kg/h intraperitoneal sodium pentobarbital (Algin, Maassluis, the Netherlands).
The cells were cultivated at 37 °C and 120 rpm and induction was carried out at an OD600 of 4. Subsequently, the cultivation was continued to an overall cultivation time of 24 h, starting from inoculation.
Subsequently, the dissection was continued upward along the ventral side of abdominal aorta.
Subsequently, the measurement was continued at a constant temperature of 25 °C for at least 3 h (strain 10−3, 6.27 rad.s−1, frequency 1 Hz).
Subsequently, analysis of samples was continued by following the same approach used for environmental samples.
Cabergoline was subsequently discontinued and DA therapy was continued using bromocriptine 5 mg/daily.
This mixture was subsequently suspended in water and polymerisation was continued to complete conversion.
The medium was subsequently changed to DMEM and incubation was continued.
Coarse aggregate was subsequently added to the rotating mixer and mixing was continued for further 2 min.
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