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To place another loxP site at the Gabrb3 locus, another replacement-type targeting vector of Gabrb3-pG12 was constructed to replace part of exon 1 and exon 2 of Gabrb3 with a cassette containing a loxP site, a puromycin selectable marker, and a 3'-half of the Hprt exon 3 to 9 minigene.
A brick house was constructed to replace their shack of mud and dung.
An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives.
In 1929, the current building, designed by Howard W. Cutler in an Italian Romanesque Revival architectural style, was constructed to replace the mission.
Additionally, an inactive PRK mutant PRK2021, which carries K20M and S21A mutations in the conserved nucleotide-binding sites of ATP-binding proteins (Higgins et al., 1986), was constructed to replace the wildtype gene in the selective plasmid.
A system of stone drains was constructed to replace the previous open ditches.
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In the 18th century, fires destroyed large parts of the city, and stone buildings were constructed to replace the old wooden houses.
In OLVFM-MOGA, the variable-fidelity metamodel (VFM) is constructed to replace the expensive simulation models to ease the computational burden.
Another station, the Jameson Canyon Pumping Station, is constructed to replace an existing installation at the Cache Slough Reservoir, which receives water from the Sacramento River system and delivers it to the City of Vallejo, California, Fleming Hill Water Treatment Plant.
A new class of carriers, the Gerald R. Ford class, is being constructed to replace previous vessels after decommissioning.
It is discovered that a new Halo is being constructed to replace the one that the Master Chief destroyed in Halo: Combat Evolved.
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