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A variant was considered conserved if it overlapped a conserved non-exonic element (CNEE) [Lowe et al., 2011] or a region present in the phastCons track from the UCSC table browser.
A sequence was considered conserved if the predicted binding affinity of its ortholog was above certain threshold (learned from the training data in Mel).
A gene was considered conserved if the TBLASTN analysis produced an alignment with a minimum of 85% identity and over between 90 to 110% of the length of the query.
A domain was considered conserved if at least two orthologous genes were still GC3 biased.
If at least half of the pairs had a BLOSUM62 value > 0, the column was considered conserved.
A binding site was considered conserved across the alignment based upon the score threshold computed with the Transfac Matrix Database (v7.0).
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Regions with scores greater than 0 are considered conserved because the observed conservation patterns fit the conserved model better than the nonconserved model.
The plate overlap pattern is considered conserved at higher taxonomic levels, and the divergent keystone Plate 3′ in Parvodinium marciniakii, sp. nov., thus appears as a striking diagnostic character.
From three hillslopes with different physical erosion rates, the concentrations of Zr, which were considered conserved during dissolution and leaching, were determined and used as a proxy for the degree of mass losses via chemical denudation.
Entire regions included in a sparse pattern were considered conserved.
We next performed DNA sequencing of parts of three additional genes (flaB, recA and ureI) that appear once in the H. pylori genome without paralogues, and the 16S rRNA gene that is present twice in the H. pylori genome and is considered conserved among all isolates of H. pylori.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com