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Moreover, a peptide YVQI in CRD2 was confirmed to bind to Src kinases, therefore regulating the phosphorylation and externalization of galectin-4 (Ideo et al., 2013).
MPEG-PLLD-Arg was confirmed to bind and deliver gene effectively, and its gene transfection efficiency was significantly higher than PEI-25k under its optimized condition.
Importantly, rFRβ was confirmed to bind to its ligand, folate.
The 35B11 clone was confirmed to bind the purified ZIP13 protein, assessed by surface plasmon resonance (SPR) experiments (Fig 4C).
1A2 was selected for binding to the surface of HT1080 cells (see scFv Library Generation) and was confirmed to bind to the surface of HT1080 and U87MG cells by immunocytochemistry, though its protein target is not known.
BAP-24 was confirmed to bind to the α-exosite, yet we wanted to further examine its value through kinetic testing in the form of mutually or nonmutually exclusive binding between BAP-24 and nonpeptidic inhibitors.
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De Groot et al. applied a bioinformatics approach for predicting HLA-B*07 restricted WNV CD8+ T cell epitopes [8]: Out of 16 predicted epitopes, 12 were confirmed to bind HLA-B*07 in vitro, but the peptides' ability to induce T-cell responses was not tested.
These scFvs were confirmed to bind to the surface of HT1080 by immunocytochemistry on live cells.
Using the recently developed GeneProf software for integrating published data sets (Halbritter et al., 2012), more than 5,000 genes were confirmed to bind Nanog in at least two independent studies.
A subset of the ZoBio internal fragment library consisting of 1,281 molecules was screened against AmpC β-lactamase using TINS, blind of the docking results, and 41 hits were confirmed to bind to AmpC in a replication experiment (hit rate 3.2%) (Supplementary Table 1).
In summary, of 304 'apparently relevant' in vivo AdpA-binding sites, 284 sites (93%) were confirmed to be bound by AdpA in vitro.
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