Exact(2)
The SjTGR entire open reading frame amplified by PCR with special primers was subcloned into the plasmid pVAX1 with T4 DNA ligase and was confirmed correct by restriction enzyme digestion and sequencing.
Briefly, initial denaturation time of 15 min was followed by 45 cycles of denaturation at 94°C for 1 minute, and annealing/extension at 60°C for 1 min. The PCR amplicon was confirmed correct by Sanger sequencing and characteristic melting temperature.
Similar(58)
The four tested methods had the same specificity for ruling out endobronchial intubation (that is, confirming correct tracheal intubation).
The insertion was confirmed to be correct by DNA sequencing.
My decision to stay in line was confirmed to be correct as I watched a couple of customers trickle out with their new iPhones.
The PCR products were inserted into the p-MIR-reporter plasmid (Ambion), and the insertion was confirmed to be correct via sequencing.
The handedness of the reconstructed volume of LDL at 37°C was confirmed to be correct by collecting tilted images at 0, 4, and 8 degrees.
One mutant named AGM1 was confirmed to be correct by PCR and Southern blot analysis.
High transformation efficiency and accuracy were achieved as ~63% of the transformants was confirmed to be correct.
After analyzing localization of these voxels and their surrounding voxels, the assignment of these voxels was confirmed to be correct.
Again, the specificity of the primers was confirmed with the correct sized PCR fragment being amplified from DNA template.
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