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Finally, the extract liquid in magnesium silicate column was concentrate to 5 mL by use of a rotary evaporator and nitrogen purge.
Toluene layer was washed with 500 mL of water containing 10 mL of 30% aqueous NaOH, toluene layer was concentrate to dryness to afford 220 g of buff white product 13.
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The released complex was concentrated to ~50 µL.
The n-butanol fraction was concentrated to dryness in vacuo.
Firstly, 2 L TOWPF was concentrated to 200 mL.
The protein was concentrated to 6 mg/mL for crystallization.
At first it was concentrated to my own take on the art world.
Elution was concentrated to a volume of 1 ml.
The protein was concentrated to 9.6 mg ml−1 for crystallization.
CoZi was concentrated to 20 mg/ml.
The eluate was concentrated to 0.5 mL.
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