A strain with quadruple CA gene deletions, Re2436 (H16∆can∆can2∆caa∆cag), was also constructed, and each CA enzyme, expressed in trans, was complemented back into the strain to create Re2436/pCan, Re2436/pCaa, Re2436/pCag and Re2436/pCan2.
The gene deletions in ΔlpcC, ΔmanB, ΔmanC and ΔkdtA strains were complemented in trans by transforming into the mutant strains plasmids that express the wild-type gene.
The pch deletion was complemented by introducing this gene into the attTn7 site (using the pUC18Tmini-Tn7T system).
Analysis of VTE prevention activity reported in 816 randomly selected orthopaedic and general medical in-patient medical records was complemented by a qualitative study into the practical responses to revised national guidance.
These observations were complemented by investigations into the connections between Stat3 and apoptosis regulators.
It is argued that the development and implementation of tools should be complemented by systematic explorations into the mechanisms that moderate the relationship between group awareness and learning.
Therefore, such mechanisms should be complemented by, and integrated into, a broader and coordinated development strategy.
Our comprehension of how adult neurogenesis is regulated begins now to be complemented with new insights into the complex epigenetic orchestration of this phenomenon, a regulatory dimension that is relatively new in neuroscience research.
Standard-issue pub food and a modest beer-and-wine selection are complemented by sixteen cocktails divided into "house" and "classic" categories.
Conventional biparametric displays have recently been complemented by new tools taking into account the multidimensional spaces integrating all characteristics of each cell studied.
