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The precipitation was washed three times with distilled water and dried to a constant weight at 45 °C as biomass, and the supernatant was collected to detect mycotoxin (citrinin).
Nasopharyngeal aspirate (NPA) was collected to detect respiratory viruses.
At different intervals after dextran addition, the medium in the low chamber was collected to detect the transported dextran by liquid scintillation counting.
Draw blood from tail vein was collected to detect blood glucose levels with a SURESTEP blood glucose meter (Lifescan) before STZ injection, every week after STZ injection, and once additionally three days after transplantation.
BALF (bronchial alveolar lavage fluid) was collected to detect KL secretion by ELISA.
The samples were centrifuged and the supernatant was collected to detect the protein concentration.
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The washing solutions were collected to detect the amount of un-immobilized GOx.
Furthermore, differentiated 3T3-L1 adipocytes were exposed to 20 μM PAQG or vehicle for 48 h and then the cell sample were collected to detect the level of p-AMPK.
Eventually, supernatants were collected to detect the expression of IFN-γ and IL-17.
Supernatants were collected to detect cytoplasmic proteins.
Furthermore, no data were collected to detect a decrease in inflammatory mediators.
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