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The FBPA fraction (retention time = 19 to 21 min) was collected by adding 25%% ascorbic acid injection and 10%% sodium chloride injection.
Immunoprecipitated chromatin was collected by adding protein G beads to the tubes and the beads were washed with ChIP-IT™ Washing Buffers supplemented with protease inhibitors.
The antibody/histone complex was collected by adding salmon sperm DNA/protein A agarose slurry (30% of the total sample volume) for 1 h at 4 °C with constant rotation.
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The bacteria were collected by adding sterilized ultrapure water and the concentration of bacteria was adjusted to 0.3 OD at 600 nm.
Then, the pyridine-capped CdSe QDs were collected by adding n-hexane to the solution and thereafter centrifuging at 4000 rpm.
After an overnight growth at 37 °C, colonies harbouring the epCelStrep encoding gene variants were collected by adding liquid LB medium containing ampicillin (100 μg/mL) to the plate surface and by suspending them with a scraper.
Nuclei were collected by adding lysis buffer after cross-linkage was stopped with glycine.
Recombinant proteins, and any bound molecules were collected by adding insoluble glutathione-Sepharose 4B (Amersham Biosciences).
After immunoprecipitation, the precipitated complexes were collected by adding 60 μL of protein A-agarose beads.
Immunoprecipitates were collected by adding protein A/G agarose (30 µl) (Santa Cruz Biotechnology, Santa Cruz, CA) for 30 minutes at 4°C.
Immunoprecipitates were collected by adding protein A/G agarose (30 µl) (Santa Cruz Biotechnology, Santa Cruz, CA) for 30 min at 4°C.
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