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Recombinant procaspase-3/9 expressed at 15 °C was cleaved to form active caspase-3/9 which has DEVDase activity.
When we checked blebbing eggs with high DEVDase activity (Fig. 3b, after 8 00), we found that procaspase-3/9 was cleaved to form caspase-3/9.
Finally, the 3' 12 bp flanking sequence in the duplex of both D-loops contained an AvaI restriction site that was cleaved to near completion confirming proper based pairing at the 3'end of the invading strand (Fig. 1C).
Following etoposide treatment, procaspase-9 was cleaved to 35 and 37 kDa fragments (p35 and p37, respectively) but TCTP did not inhibit the production of p35.
In order to characterize the conjugates between 23-mer DNA oligomer and GFP protein (Scheme 1), DNA was digested to nucleotides, while the protein was cleaved to peptides with trypsin.
The precursor protein pro-cathepsin B was cleaved to active cathepsin B by OGD in neurons (Supplementary Figure 4A) and PERK overexpression in HEK293 cells (Supplementary Figure 4B and C).
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During complement activation, C3 is cleaved to form C3a and C3b; when C3 is cleaved faster than it can be replaced, the levels of intact C3 decay.
Vitamin A, first presenting as the precursor beta-carotene, is cleaved to form retinol, which is then recombined with fatty acids before entering the chylomicron.
In the second, consecutive step the proton is shuttled to the oxygenygen and the nucleotide is being cleaved to form the product state.
Degradation is defined as the "chain-scission process, during which polymer chains are cleaved to form oligomers and, finally, to form monomers", whereas erosion "designates the loss of material owing to monomers and oligomers leaving the polymer" (Göpferich 1996).
Carotenoids can also be cleaved to form apocarotenoids.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com