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Exact(4)
The entire gene was cleaved out using HindIII and blunt-end ligated into the piggyBac-containing plasmid pIR-eGFP [8], which had been digested with XhoI and NotI.
Similarly, for the construction of pGECB that produced dimeric RecBCEc protein, 11.7 kbp DNA containing the recC-ptr-recB region of E. coli was cleaved out from the plasmid pAMP3 [54] as BamH1 fragment and then cloned into the BamH1 site of pGL10.
The 35S-GFP cassette was cleaved out with EcoRI and HindIII, and exposed to the action of several methylation-sensitive enzymes (Bsu15I, Eco72I and Eco47I).
For methylation analysis the 35S-GFP cassette was cleaved out with HindIII and EcoRI, and thereafter the DNA was subjected to methylation-sensitive restriction enzymes that cleave within the 35S-GFP cassette (Bsu15I, Eco72I, Eco47I; Fermentas), and separated on 0.8% agarose gel.
Similar(55)
Here, portions of the original translation product have to be cleaved out (inteins) and others joined together (exteins) before yielding a functional protein.
Finally, we found six novel miRNA loci potentially encoding miRNA precursors, from which 20 nt–21 nt mature miRNAs corresponding to the small RNAs in our library can be cleaved out (see Table 3 and additional file 12; additional file 13 for predicted secondary structures).
These miRNA clusters are miR-17∼92 miR-17∼923∼24∼27, the members of which and cleaved out of a polycistronic pri-miR-23∼24∼27the
Their tripartite genome consists of a small (S), a medium (M), and a large (L) fragment and the envelope glycoproteins Gn and Gc that are cleaved out of a polyprotein synthesized by the M fragment.
λ DNA was cleaved by diluted T181A.
The majority of BG molecule was cleaved within 2.5 min.
And her breast opened to it, she was cleaved like a transparent jewel to its light.
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