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The RNA product was cleaved off from CPG matrix, and the protecting group was removed with saturated ammonia at 65°C for 1 h.
Electrophoretic separation under disaggregating conditions indicated that the recombinant peptide was cleaved off by intein at the expected site and an overlay gel assay demonstrated that the peptide retained antimicrobial activity.
The H6-ZZ tag was cleaved off with Tobacco Etch Virus TEVV) protease and the 166 amino acid full-length homo-dimeric protein was purified using affinity and size-exclusion chromatography.
sCR1, a soluble form of CR1, is a recombinant protein of CR1 in which the transmembrane domain at C-terminus was cleaved off and could be over-expressed in Chinese hamster ovary (CHO) cells.
After incubation with BoNT/A-spiked serum sample for a certain time duration, the biotin at the amino terminus of the peptide was cleaved off, resulting in that gold nanoparticle-labeled streptavidin-substrate peptide complexes could not be captured on the test zone of LFTS, thus, LFTS presented a different coloration from BoNT/A-negative result (Fig. 1b).
The His-tag was cleaved off by thrombin (GE healthcare) before gel filtration.
Similar(30)
The monolith was then crosslinked by using a low-toxic polymer that could be cleaved off from the monolith hydrolytically.
The transport protein (e.g., enzyme) translates from the cytosol through the inner membrane, where the signal peptide is cleaved off by specific peptidases.
In this homologous expression system, these signal peptides were cleaved off by a signal peptidase on the N-terminal side of the arginine residue.
Contrary to previous studies, the OvEC-SOD was found to have a 19 amino acid long signal peptide that is cleaved off during the process of maturation.
These toxins contain a signal for degradation by the N-end rule pathway, which is cleaved off by HIV-1 PR, resulting in increased toxicity.
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