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Following the Me-RDA experimental design, Tester DNA was cleaved with FspBI and Driver DNA was cleaved with BfaI (see method, Figure 1A).
Pro-caspase-3 (37 kDa) was cleaved into 17 kDa fragment (cleaved caspase-3) during apoptosis.
Finally, the wafer was cleaved into laser bars and the cleaved facets were left uncoated.
However, although the 3',5'-linkage was cleaved slightly faster in Ca2+ than in Mg2+, the 2',5'-linkage was cleaved in Mg2+ (or Mn2+) but not Ca2+.
The GST tag was cleaved with PreScission protease at 4 °C overnight.
Crosslinked DNA was cleaved with 1 M pyrrolidine (Sigma) at 90 °C for 20 min.
NucS-SUMO fusion was cleaved by Ulp protease to release a native protein.
This CNBr fragment was cleaved at tryptophan residues, the subfragments were separated, and the labeled subfragments were partially sequenced.
Isolated, labeled alpha subunit was cleaved with CNBr, and the fragments were separated by reverse-phase high-performance liquid chromatography.
LONGS PEAK, the highest mountain in Rocky Mountain National Park, looks like it was cleaved with a chisel.
Loss of microstructural topography may have been due to gelatinization when collagen was cleaved by collagenase.
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