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The lysate was cleared using centrifugation at 100,000× g.
Insoluble proteins and cell fragments were removed by centrifugation (9,000 g, 25 min, 4°C) and the supernatant was cleared using a 0.45 μm filter.
The homogenate was cleared using centrifugation at 13 800 g for 15 min at 4 °C, and used for virus titre determination, cytokine profiling and caspase activation assays (described below).
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This protocol should be useful for other difficult-to-clear tissues that are unable to be cleared using classical protocols alone.
Seedlings were cleared using the method previously described [ 40].
Embryos were cleared using Murray's clear and imaged using a Leica m420 microscope with a CoolSNAP-Pro digital camera (Media cybernetics).
Only 15 100 ha were cleared using mechanical and chemical control with this substantial budget (van Wilgen et al. 2012), which makes the cost/ha very expensive (US$2828).
Within 10 - 15 min post-slaughter, blood clots in the vessels of the glands were cleared using 1 L heparinised Tyrode solution per udder half.
If it sounds cool, and can be cleared, use it.
The Catalan's message beforehand was clear: use the superb midweek win over Barça as motivation to be as focused and ruthless again.
One thing is clear, using shale instead of LNG will be no extra help for meeting the UK's own carbon targets.
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