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The coronal part of the graft was carefully fixed on the originally existing soft-tissue bridge, the apical part sits on the tissue that is denudated by vestibulum-plasty.
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Cells were carefully fixed drop-by-drop in 70% ethanol and stored at −20 °C.
The shaved and anesthetized mice (1% isoflurane, Baxter, Munich, Germany) were carefully fixed in semi-supine position supported by tamponade due to brittleness of the bones.
The brain was carefully collected and fixed in paraformaldehyde solution for 24 h followed by 30% sucrose solution for 1 week then covered with optimal cooling temperature (OCT) compound for cryostat sectioning (Ted Pella Inc., Redding, CA, USA) and stored at −80°C until histology was performed.
Limbs were dissected and soft tissue was carefully removed then fixed in formalin for 48 hours followed by 70% ethanol.
Brain tissue was carefully removed and fixed in 4% formol saline for wax embedding, and preparing tissue sections for hematoxylin and eosin staining.
After mice were placed in a supine position and the skin flap was carefully spread and fixed, intravital fluorescence imaging was conducted.
For a systematic investigation, the Au film thickness (thickness) was carefully varied while fixing the other growth parameters.
Brains were carefully removed, fixed in 4% PFA overnight at 4°C and the Circle of Willis and major arteries were examined under a microscope.
Fat bodies were carefully dissected, fixed and stained with Nile Red and documented under a fluorescence dissecting scope using a rhodamine filter.
Skeletal muscles of killed mice were carefully isolated, fixed in formalin and embedded in paraffin.
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