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The quantification of gene expression levels was calculated relative to 18S using a 2 ΔΔCT method53.
Percent reduction in infection intensity was calculated relative to the respective control IgG tested in the same assay.
Fold expression was calculated relative to the values measured for RCC4-VHL cells incubated in 20% O2.
The change in expression of each target mRNA was calculated relative to the level of 18 S rRNA44.
Fold induction of IRF1 was calculated relative to untreated (set as 1) by densitometric analysis after normalizing it to loading control β-actin.
After 4 8 h incubation at 37 °C, the diameter of the motility swarms was measured and motility was calculated relative to the wildtype control.
Fold induction of GFP-Ipr1 was calculated relative to expression in untreated cells (set as 1) by densitometric analysis after normalizing it to loading control β-actin.
Time was calculated relative to 50 K.
Expression was calculated relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Time since most recent fire (TSF) was calculated relative to 2010.
Labeling efficiency was calculated relative to the total amount of 111In added to the sample.
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