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Fold change of Ct value of each sample was calculate using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a background control.
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Prevalence was calculated using US census data.
Flap volume was calculated using dedicated software.
Volume was calculated using the Huber equation.
UPF was calculated using Eq. (3).
The IE % was calculated using Eq. (5).
Tafel slope was calculated using Eq. (1) [24].
IC50 was calculated using Sigma Plot 11.0 software.
NDVI was calculated using ENVI 5.3 software.
The average insertion loss was calculated using.
Test retest reliability was calculated using ICC.
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