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Identification of tcdC and cdtB genes was based on previous protocols (11, 24, 25 ).
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Motor neuron differentiation was based on previous protocol (33) with some modifications.
The experimental execution and processing of results were based on previous protocols (Pierce et al., 2007).
Design, construction and application of the peptide arrays were based on previous protocols with modifications [ 34].
The procedure for preparing S. aureus SA113 and E. coli Dh5α persister cells is based on previous protocols.
This postprandial timeframe for the experimental protocol was based on previous studies in rainbow trout, that had revealed a postprandial increase in hepatic expression of omy-miRNA-122b isomiRNA in rainbow trout 4 h after feeding [ 14], in addition to activation of the hepatic insulin pathway and regulation of metabolic gene expression.
The testing protocol used was based on previous studies by Horwitz et al. [29] and Egol et al. [20].
The examination protocol adopted was based on previous studies [ 16, 17].
The protocol for CEUS examinations was based on previous reports [ 6, 13, 14].
The protocol for locomotion sensitization induced by morphine was based on previous studies [ 26].
"It was based on previous relationships," Tabibian told me.
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