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Freshly hatched first instar nymphs were immobilized by exposure to -20°C for 15 min. Injection solution containing 4 μl dsRNA at 5 μg/ul concentration, 0.5 μl 10 × injection buffer (5 mM KCl, 1 mM KPO4) and 0.5 μl Phenol Red was backfilled into a needle pulled from 10 ul glass capillary tubes (Idaho Technology).
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A small mountain, a spoil heap estimated at 6,000 tonnes, stands waiting to be backfilled into the site.
It was backfilled in the 1970s with marl.
After the excavation of each opening, the opening was backfilled.
Then, the reaction flask was backfilled with Ar, and the temperature was increased to 150 °C.
The nanopipette was backfilled with buffer solution and an Ag/AgCl electrode inserted.
The street dealers they were arresting were being backfilled in less than 24 hours.
The resulting holes would be backfilled in a separate executive decree.
Then, with feverish activity from their hindquarters, the hole is backfilled.
Murder leaves a crater that must be backfilled, starting at the topsoil of the final hours.
The mine shafts were backfilled with hardcore and capped with concrete.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com