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The corresponding DNA sequence for each CEG in each species was back aligned using a custom python script (https://github.com/peterjc/pico_galaxy/tree/master/tools/align_back_trans, last accessed August 22, 2014).
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Virtual reads are generated along the whole genome and then are back aligned onto this sequence.
The simulated dinucleotide color space reads were back aligned against the S. cerevisiae reference genome using the "mapreads" program from Applied Biosystems SOLiD "corona lite 4.2.2" software package.
Afterward, as illustrated in Figure 1B, the virtual reads were back aligned against the reference genome with a given number of allowed mismatches.
In case of CDS aligning with longer or smaller proteins in the database (±10% threshold), the database protein was back-aligned to the genome sequence with tblastn.
These were aligned using Prank [ 36] and the aligned peptide sequence was back translated to generate an aligned nucleotide sequence.
The resulting images were back-projected and aligned using the IMOD package (IMOD, etomo [26], [27]) to generate the tomogram (Supplementary Movie S2).
COI sequences were aligned at the amino-acid level and back aligned to nucleotides without indels in MacClade [ 65].
If you must move the victim, be sure to keep the head, neck and back aligned.
And some at NBC still pine for Katie Couric, who, if many planets were somehow to align, could be back on a top-rated NBC morning show, albeit one that is broadcast just once a week.
Now that he's back, Ashcroft's business interests are again aligning with Tory policies.
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