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The number of CA1 uninjured neurons was assessed utilizing a stereological approach.
The specific surface area was assessed utilizing the Brunauer Emmett Teller (BET) technique, and the pore size distribution was estimated from desorption branches of nitrogen isotherms using the Barrett Joyner–Halenda (BJH) system.
The level of active Raf-bound Ras was assessed utilizing a Ras activation assay kit (Upstate, Lake Placid, NY).
The recovery cycle of excitability was assessed utilizing a paired pulse protocol, with a supramaximal stimulus followed by a test stimulus at intervals varying between 2.5 and 200 ms. Initially following conduction of an impulse, it becomes more difficult to generate a subsequent impulse due to the recovery of inactivation of transient Na+ channels [26], [27].
Viability was assessed utilizing the Live/Dead assay kit (Molecular Probes).
PLD activity was assessed utilizing a PLD transphosphatidylation reaction unique to this enzyme.
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Anxiety and depression were assessed utilizing HADS [16].
The macro- and micro-structural features, weld defects, chemical composition and mechanical property of 3.2 mm weldments were assessed utilizing optical and scanning electron microscopes equipped with EDS analysis and microhardness tester.
Deconvolved images were assessed utilizing the ImarisColoc software (Bitplane) in manual mode.
The competence of labor ward nurses providing obstetric care will be assessed utilizing case vignettes.
Oxidative stress was evaluated using Malondialdehyde (MDA) equivalents which were assessed utilizing the thiobarbituric acid (TBARS) as reactive substrate [ 35].
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